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Image Search Results
Journal: STAR Protocols
Article Title: Protocol for high-yield bacterial expression and purification of the voltage-dependent anion channel 1 for high-throughput biophysical assays
doi: 10.1016/j.xpro.2024.103557
Figure Lengend Snippet: Expected outcomes of the Fluorescence polarized assay of mVDAC1 (A) Binding of the NADH (at 5 μM) to increasing concentrations of mVDAC1 measured by fluorescence polarization (FP). The dissociation constant (KD) obtained by fitting the fluorescence polarization values and the protein concentrations into a nonlinear regression model curve in Origin Lab is about 7 μM. (B) Displacement curves of the NADH (5 μM) from mVDAC1 with increased concentrations of small-molecule D10. The molecule can compete with NADH binding with an apparent KD in the micromolar range (KD = 18.5 ± 0.2). Data are expressed as mean ± SEM ( n = 3). Reproduced from.
Article Snippet: The dissociation constant (KD) obtained by fitting the fluorescence polarization values and the protein concentrations into a
Techniques: Fluorescence, Binding Assay